Photolytic Degradation Products of Two Highly Brominated Flame Retardants Cause Cytotoxicity and mRNA Expression Alterations in Chicken Embryonic Hepatocytes

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• 作者：Guanyong Su ; Robert J. Letcher ; Doug Crump ; Reza Farmahin ; John P. Giesy ; Sean W. Kennedy
• 刊名：Environmental Science & Technology
• 出版年：2014
• 出版时间：October 21, 2014
• 年：2014
• 卷：48
• 期：20
• 页码：12039-12046
• 全文大小：300K
• ISSN：1520-5851

文摘

Tetradecabromo-1,4-diphenoxybenzene (TeDB-DiPhOBz) and 2,2鈥?3,3鈥?4,4鈥?5,5鈥?6,6鈥?decabromodiphenyl ether (BDE-209) are photolytically unstable flame retarding chemicals. Here, photocatalyzed byproducts of TeDB-DiPhOBz and BDE-209 (i.e Br₈- to Br₁₁-PB-DiPhOBz congeners from TeDB-DiPhOBz, and Br₆- to Br₈-BDE congeners from BDE-209), formed after 21 days of natural sunlight irradiation (SI), were assessed for exposure effects on cytotoxicity and mRNA expression levels of selected genes in chicken embryonic hepatocytes (CEH). CEHs were exposed for 36 h to concentrations of SI- and nonirradiated (NI)-TeDB-DiPhOBz and BDE-209. Cytotoxic effects were observed only in CEH exposed to 50 渭M SI-BDE-209. Results from a custom-designed Avian ToxChip polymerase chain reaction array showed that NI-TeDB-DiPhOBz and NI-BDE-209, up to maximum concentrations of 1.9 and 9 渭M, respectively, caused limited changes in mRNA levels of 27 genes from toxicologically relevant pathways, including phase I/II metabolism, the thyroid hormone pathway, lipid/cholesterol metabolism, oxidative stress, immune response, and cell death. In contrast, 12 and 14 of the 27 genes were altered after exposure to 25 渭M SI-TeDB-DiPhOBz or 10 渭M SI-BDE-209, respectively. Aryl hydrocarbon receptor (AhR)-related CYP1A4 mRNA levels were the most altered on the PCR array with an induction of 560- and 5200-fold after exposure to 1 or 25 渭M SI-TeDB-DiPhOBz, respectively, and 2500- and 2300-fold after exposure to 1 or 10 渭M SI-BDE-209, respectively. A dioxin-responsive luciferase reporter gene assay confirmed that the CYP1A4 inductions were independent of the dissolution solvents used (tetrahydrofuran/n-hexane, n-hexane, or methanol) during photolysis. Overall, degradation of TeDB-DiPhOBz and BDE-209 by natural sunlight generates byproducts that affect in vitro expression of genes, especially the AhR-mediated CYP1A4.