Chitosan/siRNA Nanoparticles Biofunctionalize Nerve Implants and Enable Neurite Outgrowth
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- 作者：Ursula Mittnacht ; Hanna Hartmann ; San Hein ; Hugo Oliveira ; Mingdong Dong ; Ana P. Pgo ; Jø ; rgen Kjems ; Kenneth A. Howard ; Burkhard Schlosshauer
- 刊名：Nano Letters
- 出版年：2010
- 出版时间：October 13, 2010
- 年：2010
- 卷：10
- 期：10
- 页码：3933-3939
- 全文大小：564K
- 年卷期：v.10,no.10(October 13, 2010)
- ISSN：1530-6992
文摘
Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65−75% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair.