NONOates鈥揚olyethylenimine Hydrogel for Controlled Nitric Oxide Release and Cell Proliferation Modulation

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• 作者：Jihoon Kim ; Yanggy Lee ; Kaushik Singha ; Hyun Woo Kim ; Jae Ho Shin ; Seongbong Jo ; Dong-Keun Han ; Won Jong Kim
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：June 15, 2011
• 年：2011
• 卷：22
• 期：6
• 页码：1031-1038
• 全文大小：976K
• 年卷期：v.22,no.6(June 15, 2011)
• ISSN：1520-4812

文摘

In recent years, numerous research activities have been devoted to the controlled release of nitric oxide (NO) due to its potential as a restenosis inhibitor which inhibits the proliferation of vascular smooth muscle cells, the apoptosis of vascular endothelial cells, and aggregation of platelets. This work has demonstrated the development of a novel NO-conjugated gel system comprising of thermosensitive Pluronic F127, branched polyethylenimine (BPEI), and diazeniumdiolates (NONOates). Synthesis of conjugated Pluronic-BPEI-NONOates involved coupling of activated F127 to BPEI followed by the installation of NONOates at the secondary amine sites of branched PEI backbone under high pressure. NO-conjugated gel system, F127-BPEI-NONOates, reduced the initial burst of NO release and prolonged NO release. Furthermore, F127-BPEI-NONOates polymer coated on cell culture dish displayed much higher increase of endothelial cell proliferation and reduction of smooth muscle cell proliferation than that exhibited by non-NO releasing control. Such an NO-releasing device can operate locally and has a great potential in several biomedical applications due to high biocompatibility imparted by the conjugated F127.