Differential Localization of G Protein 尾纬 Subunits

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• 作者：Katherine M. Betke ; Kristie L. Rose ; David B. Friedman ; Anthony J. Baucum ; II ; Karren Hyde ; Kevin L. Schey ; Heidi E. Hamm
• 刊名：Biochemistry
• 出版年：2014
• 出版时间：April 15, 2014
• 年：2014
• 卷：53
• 期：14
• 页码：2329-2343
• 全文大小：501K
• 年卷期：v.53,no.14(April 15, 2014)
• ISSN：1520-4995

文摘

G protein 尾纬 subunits play essential roles in regulating cellular signaling cascades, yet little is known about their distribution in tissues or their subcellular localization. While previous studies have suggested specific isoforms may exhibit a wide range of distributions throughout the central nervous system, a thorough investigation of the expression patterns of both G尾 and G纬 isoforms within subcellular fractions has not been conducted. To address this, we applied a targeted proteomics approach known as multiple-reaction monitoring to analyze localization patterns of G尾 and G纬 isoforms in pre- and postsynaptic fractions isolated from cortex, cerebellum, hippocampus, and striatum. Particular G尾 and G纬 subunits were found to exhibit distinct regional and subcellular localization patterns throughout the brain. Significant differences in subcellular localization between pre- and postsynaptic fractions were observed within the striatum for most G尾 and G纬 isoforms, while others exhibited completely unique expression patterns in all four brain regions examined. Such differences are a prerequisite for understanding roles of individual subunits in regulating specific signaling pathways throughout the central nervous system.