Convergence of Quantum Dot Barcodes with Microfluidics and Signal Processing for Multiplexed High-Throughput Infectious Disease Diagnostics

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• 作者：Jesse M. Klostranec ; Qing Xiang ; Gabriella A. Farcas ; Jeongjin A. Lee ; Alex Rhee ; Erin I. Lafferty ; Steven D. Perrault ; Kevin C. Kain ; Warren C. W. Chan
• 刊名：Nano Letters
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：7
• 期：9
• 页码：2812 - 2818
• 全文大小：578K
• 年卷期：v.7,no.9(September 2007)
• ISSN：1530-6992

文摘

Through the convergence of nano- and microtechnologies (quantum dots and microfluidics), we have created a diagnostic system capable ofmultiplexed, high-throughput analysis of infectious agents in human serum samples. We demonstrate, as a proof-of-concept, the ability todetect serum biomarkers of the most globally prevalent blood-borne infectious diseases (i.e., hepatitis B, hepatitis C, and HIV) with lowsample volume (<100 L), rapidity (<1 h), and 50 times greater sensitivity than that of currently available FDA-approved methods. We furthershow precision for detecting multiple biomarkers simultaneously in serum with minimal cross-reactivity. This device could be further developedinto a portable handheld point-of-care diagnostic system, which would represent a major advance in detecting, monitoring, treating, andpreventing infectious disease spread in the developed and developing worlds.