Enumeration of Labile Hydrogens in Natural Organic Matter by Use of Hydrogen/Deuterium Exchange Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

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• 作者：Yury Kostyukevich ; Alexey Kononikhin ; Igor Popov ; Oleg Kharybin ; Irina Perminova ; Andrey Konstantinov ; Eugene Nikolaev
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：November 19, 2013
• 年：2013
• 卷：85
• 期：22
• 页码：11007-11013
• 全文大小：467K
• 年卷期：v.85,no.22(November 19, 2013)
• ISSN：1520-6882

文摘

A method to enumerate labile hydrogens in all constituents of molecular ensemble of natural organic matter (NOM) based on our previously developed simple hydrogen/deuterium (H/D) exchange (electrospray ionization (ESI) ion source (Kostyukevich et al. Anal. Chem. 2013, 85, 5330) and ultra-high-resolution Fourier transform ion cyclotron resonance mass spectrometry is presented. The method was applied for analysis of Suwannee River fulvic acid (SRFA), which is an International Humic Substances Society standard, as well as Siberian crude oil; and lignosulfonate. We found that SRFA and lignosulfonate molecules contain 2鈥? labile hydrogens, and their number increases with the number of oxygens in the molecule. Also, we observed that compounds of Siberian crude oil ionizing in positive-ESI mode do not have labile hydrogens, while compounds ionizing in negative-ESI mode have one labile hydrogen that detaches during ESI ionization.