Metabolic Changes in Different Developmental Stages of Vanilla planifolia Pods

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• 作者：Tony Lionel Palama ; Alfi Khatib ; Young Hae Choi ; Bertrand Payet ; Isabelle Fock ; Robert Verpoorte ; Hippolyte Kodja
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：September 9, 2009
• 年：2009
• 卷：57
• 期：17
• 页码：7651-7658
• 全文大小：829K
• 年卷期：v.57,no.17(September 9, 2009)
• ISSN：1520-5118

文摘

The metabolomic analysis of developing Vanilla planifolia green pods (between 3 and 8 months after pollination) was carried out by nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis. Multivariate data analysis of the ¹H NMR spectra, such as principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA), showed a trend of separation of those samples based on the metabolites present in the methanol/water (1:1) extract. Older pods had a higher content of glucovanillin, vanillin, p-hydroxybenzaldehyde glucoside, p-hydroxybenzaldehyde, and sucrose, while younger pods had more bis[4-(β-d-glucopyranosyloxy)-benzyl]-2-isopropyltartrate (glucoside A), bis[4-(β-d-glucopyranosyloxy)-benzyl]-2-(2-butyl)tartrate (glucoside B), glucose, malic acid, and homocitric acid. A liquid chromatography−mass spectrometry (LC−MS) analysis targeted at phenolic compound content was also performed on the developing pods and confirmed the NMR results. Ratios of aglycones/glucosides were estimated and thus allowed for detection of more minor metabolites in the green vanilla pods. Quantification of compounds based on both LC−MS and NMR analyses showed that free vanillin can reach 24% of the total vanillin content after 8 months of development in the vanilla green pods.