Frequency Shifts in SERS for Biosensing

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• 作者：Kiang Wei Kho ; U. S. Dinish ; Anil Kumar ; Malini Olivo
• 刊名：ACS Nano
• 出版年：2012
• 出版时间：June 26, 2012
• 年：2012
• 卷：6
• 期：6
• 页码：4892-4902
• 全文大小：641K
• 年卷期：v.6,no.6(June 26, 2012)
• ISSN：1936-086X

文摘

We report an observation of a peculiar effect in which the vibrational frequencies of antibody-conjugated SERS-active reporter molecules are shifted in quantitative correlation with the concentration of the targeted antigen. We attribute the frequency shifts to mechanical perturbations in the antibody鈥搑eporter complex, as a result of antibody鈥揳ntigen interaction forces. Our observation thus demonstrates the potentiality of an antibody-conjugated SERS-active reporter complex as a SERS-active nanomechanical sensor for biodetection. Remarkably, our sensing scheme, despite employing only one antibody, was found to be able to achieve detection sensitivity comparable to that of a conventional sandwich immunoassay. Additionally, we have carried out a proof-of-concept study into using multiple 鈥渟tress-sensitive鈥?SERS reporters for multiplexed detection of antigen鈥揳ntibody bindings at the subdiffraction limit. The current work could therefore pave the way to realizing a label-free high-density protein nanoarray.
<h4>Keywords: h4> hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=stress&qsSearchArea=searchText">stress; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=sensor&qsSearchArea=searchText">sensor; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=SERS&qsSearchArea=searchText">SERS; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=protein&qsSearchArea=searchText">protein; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=frequency+shift&qsSearchArea=searchText">frequency shift; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=label%5C-free+biodetection&qsSearchArea=searchText">label-free biodetection