Duck-Billed Platypus Venom Peptides Induce Ca2+ Influx in Neuroblastoma Cells

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• 作者：Masaki Kita ; David StC. Black ; Osamu Ohno ; Kaoru Yamada ; Hideo Kigoshi ; Daisuke Uemura
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：December 23, 2009
• 年：2009
• 卷：131
• 期：50
• 页码：18038-18039
• 全文大小：163K
• 年卷期：v.131,no.50(December 23, 2009)
• ISSN：1520-5126

文摘

The duck-billed platypus (Ornithorhynchus anatinus) is one of the few venomous Australian mammals. We previously found that its crude venom potently induces Ca²⁺ influx in human neuroblastoma IMR-32 cells. Guided by this bioassay, we identified 11 novel peptides, including the heptapeptide H-His-Asp-His-Pro-Asn-Pro-Arg-OH (1). Compounds 1−4 and 5−11 coincided with the 6−9 N-terminal residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP) and the 132−150 part of OvCNP precursor peptide, respectively. Heptapeptide 1, which is one of the primary components of the venom fluid (200 ng/μL), induced a significant increase in [Ca²⁺]_i in IMR-32 cells at 75 μM. To the best of our knowledge, this is the first example of the isolation of the N-terminal linear fragments of CNPs in any mammal.