Single-Cell Analysis by a Scanning Thermal Lens Microscope with a Microchip: Direct Monitoring of Cytochrome c Distribution during Apoptosis Process
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- 作者：Eiichiro Tamaki ; Kiichi Sato ; Manabu Tokeshi ; Kae Sato ; Makoto Aihara ; and Takehiko Kitamori
- 刊名：Analytical Chemistry
- 出版年：2002
- 出版时间：April 1, 2002
- 年：2002
- 卷：74
- 期：7
- 页码：1560 - 1564
- 全文大小：371K
- 年卷期：v.74,no.7(April 1, 2002)
- ISSN：1520-6882
文摘
We developed a microsystem for cell experiments consisting of a scanning thermal lens microscope detectionsystem and a cell culture microchip. The microchipsystem was good for liquid control in microspace, and thisresults in secure cell stimulation and coincident in vivoobservation of the cell responses. The system could detectnonfluorescent biological substances with extremely highsensitivity without any labeling materials and had a highspatial resolution of ~1 
m. This system was applied tomonitoring of cytochrome c distribution in a neuroblastoma-glioma hybrid cell cultured in the microflask (1 mm× 10 mm × 0.1 mm; 1 L) fabricated in a glass microchip. Cytochrome c release from mitochondria to cytosolduring the apoptosis process was successfully monitoredwith this system. The cytochrome c detected with thissystem was estimated to be ~10 zmol. We concluded thatthe system was suitable for measuring the distribution ofchemical substances in a single cell because the microchip is good for liquid handling in microspace and thethermal lens microscope has high sensitivity and spatialresolution.
m. This system was applied tomonitoring of cytochrome c distribution in a neuroblastoma-glioma hybrid cell cultured in the microflask (1 mm× 10 mm × 0.1 mm; 1 L) fabricated in a glass microchip. Cytochrome c release from mitochondria to cytosolduring the apoptosis process was successfully monitoredwith this system. The cytochrome c detected with thissystem was estimated to be ~10 zmol. We concluded thatthe system was suitable for measuring the distribution ofchemical substances in a single cell because the microchip is good for liquid handling in microspace and thethermal lens microscope has high sensitivity and spatialresolution.