Lyngbyatoxin Biosynthesis: Sequence of Biosynthetic Gene Cluster and Identification of a Novel Aromatic Prenyltransferase

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• 作者：Daniel J. Edwards and William H. Gerwick
• 刊名：Journal of the American Chemical Society
• 出版年：2004
• 出版时间：September 22, 2004
• 年：2004
• 卷：126
• 期：37
• 页码：11432 - 11433
• 全文大小：46K
• 年卷期：v.126,no.37(September 22, 2004)
• ISSN：1520-5126

文摘

The lyngbyatoxins are potent skin irritants produced by Lyngbya majuscula and cause a condition known as "Swimmer's Itch" off Honolulu, HI. Reported is the molecular cloning of the lyngbyatoxin (ltx) biosynthetic gene cluster from L. majuscula using a strategy based on its predicted nonribosomal peptide synthetase (NRPS) assembly. The biosynthetic gene cluster spans 11.3 kilobase pairs and encodes for a two-module NRPS (LtxA), a P450 monooxygenase (LtxB), an aromatic prenyltransferase (LtxC), and an oxidase/reductase protein (LtxD). LtxC was heterologously produced and purified from E. coli and shown to catalyze the transfer of a geranyl group to (-)-indolactam V as the final step in the biosynthesis of lyngbyatoxin A.