Reconstitution of Human Ether-a-go-go-Related Gene Channels in Microfabricated Silicon Chips

详细信息    查看全文

• 作者：Azusa Oshima ; Ayumi Hirano-Iwata ; Hideki Mozumi ; Yutaka Ishinari ; Yasuo Kimura ; Michio Niwano
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：May 7, 2013
• 年：2013
• 卷：85
• 期：9
• 页码：4363-4369
• 全文大小：386K
• 年卷期：v.85,no.9(May 7, 2013)
• ISSN：1520-6882

文摘

This paper reports on the reconstitution of human ether-a-go-go-related gene (hERG) channels in artificial bilayer lipid membranes (BLMs) formed in micropores fabricated in silicon chips. The hERG channels were isolated from Chinese hamster ovary cell lines expressing the channels and incorporated into the BLMs formed by a process in which the two lipid monolayers were folded into the micropores. The characteristic features of hERG channels reported by the patch-clamp method, including single-channel conductance, voltage dependence, sensitivity to typical drugs and dependence on the potassium concentration, were investigated in the BLM reconstitution system. The BLM with hERG channels incorporated exhibited a lifetime of 65 h and a tolerance to repetitive solution exchanges. Such stable BLMs containing biological channels have the potential for use in a variety of applications, including high-throughput drug screening for various ion-channel proteins.