Dimyristoyl Phosphatidylcholine: A Remarkable Exception to α-Tocopherol’s Membrane Presence
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- 作者：Drew Marquardt ; Justin A. Williams ; Jacob J. Kinnun ; Norbert Ku?erka ; Jeffrey Atkinson ; Stephen R. Wassall ; John Katsaras ; Thad A. Harroun
- 刊名：Journal of the American Chemical Society
- 出版年：2014
- 出版时间：January 8, 2014
- 年：2014
- 卷：136
- 期：1
- 页码：203-210
- 全文大小：476K
- ISSN：1520-5126
文摘
Using data obtained from different physical techniques (i.e., neutron diffraction, NMR and UV spectroscopy), we present evidence which explains some of the conflicting and inexplicable data found in the literature regarding 伪-tocopherol鈥檚 (aToc鈥檚) behavior in dimyristoyl phosphatidylcholine (di-14:0PC) bilayers. Without exception, the data point to aToc鈥檚 active chromanol moiety residing deep in the hydrophobic core of di-14:0PC bilayers, a location that is in stark contrast to aToc鈥檚 location in other PC bilayers. Our result is a clear example of the importance of lipid species diversity in biological membranes and importantly, it suggests that measurements of aToc鈥檚 oxidation kinetics, and its associated byproducts observed in di-14:0PC bilayers, should be reexamined, this time taking into account its noncanonical location in this bilayer.