Polydiacetylene Liposomal Aequorin Bioluminescent Device for Detection of Hydrophobic Compounds

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• 作者：Ryoko Yamamoto ; Shigehiko Takegami ; Atsuko Konishi ; Hikari Horikawa ; Sayumi Yonezawa ; Tatsuya Kitade
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 7, 2016
• 年：2016
• 卷：88
• 期：11
• 页码：5704-5709
• 全文大小：343K
• 年卷期：0
• ISSN：1520-6882

文摘

In this study, a polydiacetylene liposomal aequorin bioluminescent device (PLABD) that functioned through control of the membrane transport of Ca²⁺ ions was developed for detecting hydrophobic compounds. In the PLABD, aequorin was encapsulated in an internal water phase and a calcium ionophore (CI) was contained in a hydrophobic region. Membrane transport of Ca²⁺ ions across the CI was suppressed by polymerization between diacetylene molecules. On addition of an analyte, the membrane transport of Ca²⁺ ions across the CI increased, and Ca²⁺ ions from the external water phase could diffuse into the internal water phase via the CI, which resulted in bioluminescence of the aequorin. Lidocaine, procaine, and procainamide were used as model compounds to test the validity of the detection mechanism of the PLABD. When each analyte was added to a suspension of the PLABD, bioluminescence from the aequorin in the PLABD was observed, and the level of this bioluminescence increased with increasing analyte concentration. There was a linear relationship between the logarithm of the analyte concentration and the bioluminescence for all analytes as follows: R = 0.89 from 10 nmol L^–1 to 10 mmol L^–1 for lidocaine, R = 0.66 from 10 nmol L^–1 to 100 μmol L^–1 for procaine, and R = 0.74 from 100 nmol L^–1 to 100 μmol L^–1 for procainamide. Compared to the traditional colorimetric method using polydiacetylene liposome, the PLABD was superior for both the sensitivity and dynamic range. Thus, PLABD is a valid, simple, and sensitive signal generator for detection of hydrophobic compounds that interact with PLABD membranes.