Biochemical Characterization of Soybean Protein Consisting of Different Subunits of Glycinin
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- 作者：Kazuhiro Yagasaki ; Toshio Takagi ; Miyo Sakai ; and Keisuke Kitamura
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1997
- 出版时间：March 1997
- 年：1997
- 卷：45
- 期：3
- 页码：656 - 660
- 全文大小：296K
- 年卷期：v.45,no.3(March 1997)
- ISSN：1520-5118
文摘
Soybean isogenic lines having different glycinin subunit compositionsare now being bred. Tocompare the characteristics of the genetically improved soybeanproteins, total protein and purifiedglycinin proteins were investigated in this study. The absence ofsubunit(s) resulted in markedchanges in the proportion of glycinin to 
-conglycinin. When thepH of the total protein and glycininsolution was changed and NaCl was added to the solution, glycininconsisting of only group I subunitsand -conglycinin are highly soluble compared to theA5A4B3 orA3B4 subunit at a NaClconcentrationlower than 0.05 M at pH 5.5. Ultracentrifuge analysis showed thatthe glycinin containing allsubunits was able to assemble to form the 11S structure. Thatconsisting of group I subunits formeda uniform 7S structure. Decreasing glycinin/-conglycinin ratiosand structural changes caused bya lack of glycinin subunit(s) are thus expected to influence thefood-processing properties of soybeans.Keywords: Soybean; glycinin; mutant; subunit composition
-conglycinin. When thepH of the total protein and glycininsolution was changed and NaCl was added to the solution, glycininconsisting of only group I subunitsand -conglycinin are highly soluble compared to theA5A4B3 orA3B4 subunit at a NaClconcentrationlower than 0.05 M at pH 5.5. Ultracentrifuge analysis showed thatthe glycinin containing allsubunits was able to assemble to form the 11S structure. Thatconsisting of group I subunits formeda uniform 7S structure. Decreasing glycinin/-conglycinin ratiosand structural changes caused bya lack of glycinin subunit(s) are thus expected to influence thefood-processing properties of soybeans.Keywords: Soybean; glycinin; mutant; subunit composition