Benzo[a]pyrene-Induced Changes in MicroRNA鈥搈RNA Networks
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- 作者：Daneida Lizarraga ; Stan Gaj ; Karen J. Brauers ; Leen Timmermans ; Jos C. Kleinjans ; Joost H. M. van Delft
- 刊名：Chemical Research in Toxicology
- 出版年：2012
- 出版时间：April 16, 2012
- 年：2012
- 卷：25
- 期：4
- 页码：838-849
- 全文大小：773K
- 年卷期：v.25,no.4(April 16, 2012)
- ISSN：1520-5010
文摘
Toxicological studies assessing the safety of compounds for humans frequently use in vitro systems to characterize toxic responses in combination with transcriptomic analyses. Thus far, changes have mostly been investigated at the mRNA level. Recently, microRNAs have attracted attention because they are powerful negative regulators of mRNA levels and, thus, may be responsible for the modulation of important mRNA networks implicated in toxicity. This study aimed to identify possible microRNA鈥搈RNA networks as novel interactions on the gene expression level after a genotoxic insult. We used benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, as a model genotoxic/carcinogenic compound. We analyzed time-dependent effects on mRNA and microRNA profiles in HepG2 cells, a widely used human liver cell line that expresses active p53 and is competent for the biotransformation of BaP. Changes in microRNA expression in response to BaP, in combination with multiple alterations of mRNA levels, were observed. Many of these altered mRNAs are targets of altered microRNAs. Using pathway analysis, we evaluated the relevance of such microRNA deregulations to genotoxicity. This revealed eight microRNAs that appear to participate in specific BaP-responsive pathways relevant to genotoxicity, such as apoptotic signaling, cell cycle arrest, DNA damage response, and DNA damage repair. Our results particularly highlight the potential of microRNA-29b, microRNA-26a-1*, and microRNA-122* as novel players in the BaP response. Therefore, this study demonstrates the added value of an integrated microRNA鈥搈RNA approach for identifying molecular mechanisms induced by BaP in an in vitro human model.