Development and Application of an Electron Spin Resonance Spectrometry Method for the Determination of Oxygen Free Radical Formation by Particulate Matter

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• 作者：Jacob J. Bried&eacute ; Theo M. C. M. de Kok ; Janneke G. F. Hogervorst ; Edwin J. C. Moonen ; Clif L. B. op den Camp ; and Jos C. S. Kleinjans
• 刊名：Environmental Science & Technology
• 出版年：2005
• 出版时间：November 1, 2005
• 年：2005
• 卷：39
• 期：21
• 页码：8420 - 8426
• 全文大小：225K
• 年卷期：v.39,no.21(November 1, 2005)
• ISSN：1520-5851

文摘

Exposure to increased levels of ambient particulatematter (PM) are associated with several health effects,including cardiopulmonary diseases. The formation of reactiveoxygen species (ROS) is thought to play an importantrole in the induction of these health effects. To quantifythe ROS generating capacity of PM, we developed an improvedelectron spin resonance (ESR) spectrometry-basedmethod. ROS formation was measured directly on PM-containing filters, thereby avoiding the selective extractionof components and loss of material or reactivity, whichis likely to occur during filter extraction. Also, ascorbic acidwas added to stimulate ROS formation. This method wasapplied to PM₁₀ samples originating from different sources.The radical generating capacity of PM₁₀ from bothgasoline and diesel engine exhaust was significantlyhigher as compared to that of PM₁₀ from ambient or indoorair. Furthermore, in urban PM₁₀ and PM_2.5, ROS-generatingcapacity significantly correlated with concentrations ofpolycyclic aromatic hydrocarbon content and particulartransition metals. This indicates that this improved ESR methodmay be a valuable tool for evaluating the relationshipbetween ROS formation by PM and the adverse healtheffects associated with this type of air pollution.