Synchrotron FTIR Microspectroscopy of Single Natural Silk Fibers

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• 作者：Shengjie Ling ; Zeming Qi ; David P. Knight ; Zhengzhong Shao ; Xin Chen
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：September 12, 2011
• 年：2011
• 卷：12
• 期：9
• 页码：3344-3349
• 全文大小：902K
• 年卷期：v.12,no.9(September 12, 2011)
• ISSN：1526-4602

文摘

Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTIR the 尾-sheet content in a range of natural single silk fibers, 28 卤 4, 23 卤 2, and 17 卤 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of 尾-sheet content in different silk fibers from the current study accords quite well with published data determined by XRD, Raman, and p>13p>C NMR. Our results indicate that the S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers.