Microbial Diversity and Changes in the Distribution of Dehalogenase Genes during Dechlorination with Different Concentrations of cis-DCE
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- 作者：Kotaro Ise ; Koichi Suto ; Chihiro Inoue
- 刊名：Environmental Science & Technology
- 出版年：2011
- 出版时间：June 15, 2011
- 年：2011
- 卷：45
- 期：12
- 页码：5339-5345
- 全文大小：850K
- 年卷期：v.45,no.12(June 15, 2011)
- ISSN：1520-5851
文摘
A dechlorinating consortium (designated as TES-1 culture) able to convert trichloroethene (TCE) to ethene was established from TCE-contaminated groundwater. This culture had the ability of complete dechlorination of TCE within about one month. From the clone library analysis of 16S rRNA gene, this culture was mainly composed of fermentation bacteria, such as Clostridium spp., and Desulfitobacterium spp. known as facultative dechlorinator. PCR using specific primers for Dehalococcoides spp. and the dehalogenase genes confirmed that the culture contained the Dehalococcoides spp. 16S rRNA gene and three dehalogenase genes, tceA, vcrA and bvcA. Dechlorination experiments using cis-dichloroethene (cis-DCE) at concentrations of 37鈥?46 渭M, revealed that the gene copy numbers of tceA, vcrA, and bvcA increased up to 107 copy/mL, indicating that Dehalococcoides spp. containing these three dehalogenase genes were involved in cis-DCE dechlorination. However, in the culture to which 292 渭M of cis-DCE was added, only the tceA gene and the Dehalococcoides spp. 16S rRNA gene increased up to 107 copy/mL. The culture containing 292 渭M of cis-DCE also exhibited about one tenth slower ethene production rate compared to the other cultures.