Electronic Detection of Lectins Using Carbohydrate-Functionalized Nanostructures: Graphene versus Carbon Nanotubes
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- 作者：Yanan Chen ; Harindra Vedala ; Gregg P. Kotchey ; Aymeric Audfray ; Samy Cecioni ; Anne Imberty ; S茅bastien Vidal ; Alexander Star
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：January 24, 2012
- 年：2012
- 卷：6
- 期：1
- 页码：760-770
- 全文大小：1193K
- 年卷期：v.6,no.1(January 24, 2012)
- ISSN：1936-086X
文摘
Here we investigated the interactions between lectins and carbohydrates using field-effect transistor (FET) devices comprised of chemically converted graphene (CCG) and single-walled carbon nanotubes (SWNTs). Pyrene- and porphyrin-based glycoconjugates were functionalized noncovalently on the surface of CCG-FET and SWNT-FET devices, which were then treated with 2 渭M nonspecific and specific lectins. In particular, three different lectins (PA-IL, PA-IIL, and ConA) and three carbohydrate epitopes (galactose, fucose, and mannose) were tested. The responses of 36 different devices were compared and rationalized using computer-aided models of carbon nanostructure/glycoconjugate interactions. Glycoconjugate surface coverage in addition to one-dimensional structures of SWNTs resulted in optimal lectin detection. Additionally, lectin titration data of SWNT- and CCG-based biosensors were used to calculate lectin dissociation constants (Kd) and compare them to the values obtained from the isothermal titration microcalorimetry technique.