A Highly Selective Fluorescent Chemosensor for Zinc Ion and Imaging Application in Living Cells

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• 作者：Uday Chand Saha ; Basab Chattopadhyay ; Koushik Dhara ; Sushil Kumar Mandal ; Sandipan Sarkar ; Anisur Rahman Khuda-Bukhsh ; Monika Mukherjee ; Madeleine Helliwell ; Pabitra Chattopadhyay
• 刊名：Inorganic Chemistry
• 出版年：2011
• 出版时间：February 21, 2011
• 年：2011
• 卷：50
• 期：4
• 页码：1213-1219
• 全文大小：953K
• 年卷期：v.50,no.4(February 21, 2011)
• ISSN：1520-510X

文摘

A new 2,6-bis(5,6-dihydrobenzo[4,5]imidazo[1,2-c]quinazolin-6-yl)-4-methylphenol (1) serves as a highly selective and sensitive fluorescent probe for Zn²⁺ in a HEPES buffer (50 mM, DMSO:water = 1:9 (v/v), pH = 7.2) at 25 掳C. The increase in fluorescence in the presence of Zn²⁺ is accounted for by the formation of dinuclear Zn²⁺ complex [Zn₂(C₃₅H₂₅N₆O)(OH)(NO₃)₂(H₂O)] (2), characterized by X-ray crystallography. The fluorescence quantum yield of the chemosensor 1 is only 0.019, and it increases more than 12-fold (0.237) in the presence of 2 equiv of the zinc ion. Interestingly, the introduction of other metal ions causes the fluorescence intensity to be either unchanged or weakened. By incubation of cultured living cells (A375 and HT-29) with the chemosensor 1, intracellular Zn²⁺ concentrations could be monitored through selective fluorescence chemosensing.