Highly Flexible UV鈥揤is Radiation Sources and Novel Detection Schemes for Spectrophotometric HPLC Detection

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• 作者：Karsten G. Kraiczek ; R. Bonjour ; Y. Salvad茅 ; Roland Zengerle
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：January 21, 2014
• 年：2014
• 卷：86
• 期：2
• 页码：1146-1152
• 全文大小：412K
• ISSN：1520-6882

文摘

The concept and performance of the first multiwavelength deep UV light-emitting-diode-based high-performance liquid chromatography (HPLC) absorbance detector are presented. In single-wavelength mode and with optical reference, the limit of detection (LOD) is comparable to conventional state-of-the-art HPLC absorbance detectors. In multiwavelength mode鈥攁t present up to eight wavelengths without optical reference鈥攖he LOD is about 10 times higher than in single-wavelength mode. Multiplexing and demultiplexing methods are used to separate chromatographic signals in multiwavelength mode and keeps the detector configuration simple and yet flexible. Depending on the operation mode, stray light is either totally negligible or controlled electronically and digitally.