Gold Nanoparticle Based FRET Asssay for the Detection of DNA Cleavage

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• 作者：Paresh Chandra Ray ; Angela Fortner ; Gopala Krishna Darbha
• 刊名：Journal of Physical Chemistry B
• 出版年：2006
• 出版时间：October 26, 2006
• 年：2006
• 卷：110
• 期：42
• 页码：20745 - 20748
• 全文大小：91K
• 年卷期：v.110,no.42(October 26, 2006)
• ISSN：1520-5207

文摘

We report gold nanoparticle based FRET assay to monitor the cleavage of DNA by nucleases. Fluorescencesignal enhancement is observed by a factor of 120 after the cleavage reaction in the presence of S1 nuclease.The mechanism of distant dependent fluorescence quenching has been discussed. Our experimental resultson distance dependent fluorescence quenching match quite well with theoretical findings obtained from thefluorescence quenching model by Gersten and Nitzan (Surf. Sci. 1985, 158, 165). Our experimental observationparadigm for the design of optical based molecular ruler strategies at distances more than double the distancesachievable using traditional dipole-dipole Columbic energy transfer based methods.