Proteomic Studies of the Intrinsically Unstructured Mammalian Proteome
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- 作者：Charles A. Galea ; Vishwajeeth R. Pagala ; John C. Obenauer ; Cheon-Gil Park ; Clive A. Slaughter ; Richard W. Kriwacki
- 刊名：Journal of Proteome Research
- 出版年：2006
- 出版时间：October 2006
- 年：2006
- 卷：5
- 期：10
- 页码：2839 - 2848
- 全文大小：571K
- 年卷期：v.5,no.10(October 2006)
- ISSN：1535-3907
文摘
Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved incellular signaling and regulation. The aim of this study was to develop methodology for the enrichmentand identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98
C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signalingor regulation. These studies represent the first large-scale experimental investigation of the intrinsicallyunstructured mammalian proteome.
C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signalingor regulation. These studies represent the first large-scale experimental investigation of the intrinsicallyunstructured mammalian proteome.