Characterization of DNA Hybridization on Partially Aminated Diamond by Aromatic Compounds

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• 作者：Jung-Hoon Yang ; Kwang-Soup Song ; Guo-Jun Zhang ; Munenori Degawa ; Yoshinori Sasaki ; Iwao Ohdomari ; Hiroshi Kawarada
• 刊名：Langmuir
• 出版年：2006
• 出版时间：December 19, 2006
• 年：2006
• 卷：22
• 期：26
• 页码：11245 - 11250
• 全文大小：399K
• 年卷期：v.22,no.26(December 19, 2006)
• ISSN：1520-5827

文摘

Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partiallyamino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probeoligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigatedby X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group ondiamond for probe oligonucleotides, three kinds of dicarboxylic compound-adipic acid, terephthalic acid, and trimesicacid-were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridizedwith target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensitiesdetermined by epifluorescence microscopy were compared and analyzed.