The Membrane-Dipped Neuronal SNARE Complex: A Site-Directed Spin Labeling Electron Paramagnetic Resonance Study

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• 作者：Dae-Hyuk Kweon ; Chang Sup Kim ; and Yeon-Kyun Shin
• 刊名：Biochemistry
• 出版年：2002
• 出版时间：July 23, 2002
• 年：2002
• 卷：41
• 期：29
• 页码：9264 - 9268
• 全文大小：61K
• 年卷期：v.41,no.29(July 23, 2002)
• ISSN：1520-4995

文摘

The formation of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE) complex is an essential process for membrane fusion and the neurotransmitter release in neurons.As an initial step toward the determination of the membrane topology of the SNARE complex, residuesat the membrane-water interface were investigated with site-specific spin labeling electron paramagneticresonance. EPR analysis revealed that the basic amino acid-rich interfacial region, which is universal forall transmembrane SNARE proteins, inserts into the membrane, eliminating the gap between the corecomplex and the membrane. The result raises the possibility that core complex formation directly leadsto the apposition of two membranes, which could facilitate membrane fusion.