Synthesis of a Barbell-like Triblock Copolymer, Poly(L-lysine) Dendrimer-block-Poly(ethylene glycol)-block-Poly(L-lysine) Dendrimer, and Its
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- 作者：Joon Sig Choi ; Dong Kyoon Joo ; Chang Hwan Kim ; Kwan Kim ; and Jong Sang Park
- 刊名：Journal of the American Chemical Society
- 出版年：2000
- 出版时间：January 26, 2000
- 年：2000
- 卷：122
- 期：3
- 页码：474 - 480
- 全文大小：233K
- 年卷期：v.122,no.3(January 26, 2000)
- ISSN：1520-5126
文摘
A barbell-like ABA-type triblock copolymer, poly(L-lysine) dendrimer-block-poly(ethylene glycol)-block-poly(L-lysine) dendrimer (PLLD-PEG-PLLD), was synthesized by the liquid-phase peptide synthesismethod. The self-assembling complex formation of the third and fourth generation of the copolymer withplasmid DNA was studied. 1H NMR and matrix-assisted laser desorption/ionization-time-of-flight massspectrometry (MALDI-TOF MS) were used for the characterization of the synthesized copolymer. The self-assembling behavior of the 3rd and 4th generations of the copolymer with plasmid DNA was investigated byelectrophoretic mobility shift assay, DNase I protection assay, and ethidium bromide exclusion assay. Weobserved great differences in the self-assembling ability of the 3rd and 4th generations of the polymer. Thissuggests that the number of positively charged amines per polymer molecule should be an important factor forthe potential for self-assembling complex formation with DNA. Atomic force microscopy (AFM) and 
potentialswere used for evaluating the shape, size distribution, and surface charge of the complexes at various chargeratios. From AFM images, it was observed that the shape of the complex was nearly spherical and its size wasabout 50-150 nm in diameter. The in vitro cytotoxicity of the copolymer was compared with that of poly(L-lysine), poly(D-lysine), and polyethylenimine.
potentialswere used for evaluating the shape, size distribution, and surface charge of the complexes at various chargeratios. From AFM images, it was observed that the shape of the complex was nearly spherical and its size wasabout 50-150 nm in diameter. The in vitro cytotoxicity of the copolymer was compared with that of poly(L-lysine), poly(D-lysine), and polyethylenimine.