Polyozellin Isolated from Polyozellus multiplex Induces Phase 2 Enzymes in Mouse Hepatoma Cells and Differentiation in Human Myeloid Leukaemic Cell Lines
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- 作者：Jeong Hyun Kim ; Jeong Soon Lee ; Kyung-Sik Song ; Chong-Suk Kwon ; Young Kyoon Kim ; and Jong-Sang Kim
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：February 11, 2004
- 年：2004
- 卷：52
- 期：3
- 页码：451 - 455
- 全文大小：108K
- 年卷期：v.52,no.3(February 11, 2004)
- ISSN：1520-5118
文摘
Induction of cellular phase 2 detoxifying enzymes is associated with cancer preventive potential.Quinone reductase (QR) has been used as a prototype for anticarcinogenic phase 2 enzymes becauseof its widespread distribution in mammalian systems, large amplitude of inducer response, and easeof measurement in murine hepatoma cells. Methanol extract of Polyozellus multiplex, which showsa strong QR induction potential, was subjected to bioassay-guided fractionation, and polyozellin (PM1)appeared to be a major active component. In in vitro cultured cells (hepa1c1c7 and BPRc1 cells),polyozellin enhanced QR, glutathione S-transferase (GST) activities, and glutathione (GSH) contentin a dose-dependent manner. The compound also significantly promoted differentiation of HL-60human promyelocytic emia cells. In conclusion, polyozellin deserves further in vivo study to evaluateits potential as a cancer preventive agent.Keywords: Glutathione-S-transferase; quinone reductase; polyozellin; cancer prevention