Distinct BimBH3 (BimSAHB) Stapled Peptides for Structural and Cellular Studies

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• 作者：Greg H. Bird ; Evripidis Gavathiotis ; James L. LaBelle ; Samuel G. Katz ; Loren D. Walensky
• 刊名：ACS Chemical Biology
• 出版年：2014
• 出版时间：March 21, 2014
• 年：2014
• 卷：9
• 期：3
• 页码：831-837
• 全文大小：520K
• 年卷期：v.9,no.3(March 21, 2014)
• ISSN：1554-8937

文摘

Hydrocarbon stapling is a chemical approach to restoring and fortifying the natural 伪-helical structure of peptides that otherwise unfold when taken out of context from the host protein. By iterating the peptide sequence, staple type, and sites of insertion, discrete compositions can be generated to suit a diversity of biochemical, structural, proteomic, cellular, and drug development applications. Here, we reinforce key design considerations to avoid pitfalls and maximize progress when applying stapled peptides in chemistry and biology research.