Cationic Detergent/Sterol Mixtures Can Form Fluid Lamellar Phases and Stable Unilamellar Vesicles

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• 作者：Thida Phoeung ; Liliana Morfin Huber ; Michel Lafleur
• 刊名：Langmuir
• 出版年：2009
• 出版时间：May 19, 2009
• 年：2009
• 卷：25
• 期：10
• 页码：5778-5784
• 全文大小：815K
• 年卷期：v.25,no.10(May 19, 2009)
• ISSN：1520-5827

文摘

In recent studies, it has been shown that mixtures of palmitic acid (PA), and cholesterol or cholesterol sulfate (Schol), in a PA/sterol molar ratio of 30/70 lead to the formation of liquid-ordered (lo) lamellar phases. The extrusion of these systems gave large unilamellar vesicles (LUVs) that displayed a very limited passive permeability, a property associated with their high sterol content. In this study, we showed that the formation of lo-phase bilayers was also possible when mixing a cationic detergent (cetylpyridinium chloride, CPC) and sterol in a 30/70 molar ratio. The existence of this phase was established using IR and ²H NMR spectroscopy. Moreover, ²H NMR allowed us to study the orientation and dynamics of CPC and cholesterol in these self-assemblies. The extrusion of the CPC/Schol bilayers leads to the formation of LUVs, and their passive permeability was found to be very limited, making them interesting candidates as nanovectors.