Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers

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• 作者：Manoj Lakshmanan ; Yutaka Kodama ; Takeshi Yoshizumi ; Kumar Sudesh ; Keiji Numata
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：January 14, 2013
• 年：2013
• 卷：14
• 期：1
• 页码：10-16
• 全文大小：398K
• 年卷期：v.14,no.1(January 14, 2013)
• ISSN：1526-4602

文摘

To develop a new easy and quick gene delivery system for any types of plants, we prepared ionic complexes of plasmid DNA with designed peptide carriers, each of which combined a cell-penetrating peptide (Bp100 or Tat₂) with a polycation (nona-arginine or a copolymer of histidine and lysine). The present system via the designed peptides demonstrated rapid and efficient transient transfections into intact leaf cells of Nicotiana benthamiana and Arabidopsis thaliana without protoplast preparations. The designed peptides demonstrated significantly higher transfection efficiency in comparison to the nonfusion peptides (Bp100, Tat2, nona-arginine, and copolymer of histidine and lysine), indicating that the combination of functional peptides was a key to develop an efficient peptide-based gene delivery system. On the basis of the results, we exhibited the versatility of the designed peptide-based gene delivery system, which will explore the application of plant biotechnology.