Wheat Bran Oil and Its Fractions Inhibit Human Colon Cancer Cell Growth and Intestinal Tumorigenesis in Apcmin/+ Mice

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• 作者：Shengmin Sang ; Jihyeung Ju ; Joshua D. Lambert ; Yong Lin ; Jungil Hong ; Mousumi Bose ; Steven Wang ; Naisheng Bai ; Kan He ; Bandaru S. Reddy ; Chi-Tang Ho ; Frederick Li ; Chung S. Yang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：December 27, 2006
• 年：2006
• 卷：54
• 期：26
• 页码：9792 - 9797
• 全文大小：111K
• 年卷期：v.54,no.26(December 27, 2006)
• ISSN：1520-5118

文摘

This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. Westudied the colon cancer preventive effects of WB oil and its subfractions in the Apc^min/+ mouse model,a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines(HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oilfraction of WB was more active than the water fraction against the growth of human colon cancercell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001)in the Apc^min/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicalsand the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk(en)ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography.Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growthinhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in theApc^min/+ mouse model. The current results demonstrate, for the first time, the intestinal cancerpreventive activity of WB oil. The active ingredients, however, remain to be identified.