Molecular Effects of Baicalein in Hct116 Cells and Caenorhabditis elegans: Activation of the Nrf2 Signaling Pathway and Prolongation of Lifespan
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- 作者：Susannah Havermann ; Ricarda Rohrig ; Yvonni Chovolou ; Hans-Ulrich Humpf ; Wim W盲tjen
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：March 6, 2013
- 年：2013
- 卷：61
- 期：9
- 页码：2158-2164
- 全文大小：310K
- 年卷期：v.61,no.9(March 6, 2013)
- ISSN：1520-5118
文摘
Baicalein is a major compound of extracts derived from Scutellaria baicalensis Lamiaceae, which are used as food supplements. Baicalein possesses a high radical scavenging activity and decreases intracellular reactive oxygen species in Hct116 human colon carcinoma cells and in Caenorhabditis elegans. It activates Nrf2, a key transcription factor that binds to the antioxidant responsive element (ARE): Baicalein causes a nuclear accumulation of Nrf2, increases ARE-dependent luciferase activity, and enhances the expression of heme oxygenase-1 in Hct116 cells. Additionally, accumulation of the Nrf2 homologue SKN-1 in nuclei of intestinal cells of C. elegans was observed. Lifespan analysis revealed that baicalein extends the mean, median, and maximum lifespans of the nematode by 45, 57 and 24%, respectively. Because SKN-1 activation is associated with prolongation of lifespan, the results suggest that baicalein increases the lifespan of C. elegans by activation of the Nrf2/SKN-1 signaling pathway.