Gene Expression Differences between Noccaea caerulescens Ecotypes Help to Identify Candidate Genes for Metal Phytoremediation

详细信息    查看全文

• 作者：Pauliina Halimaa ; Ya-Fen Lin ; Viivi H. Ahonen ; Daniel Blande ; Stephan Clemens ; Attila Gyenesei ; Elina H盲iki枚 ; Sirpa O. K盲renlampi ; Asta Laiho ; Mark G. M. Aarts ; Juha-Pekka Pursiheimo ; Henk Schat ; Holger Schmidt ; Marjo H. Tuomainen ; Arja I. Tervahauta
• 刊名：Environmental Science & Technology
• 出版年：2014
• 出版时间：March 18, 2014
• 年：2014
• 卷：48
• 期：6
• 页码：3344-3353
• 全文大小：389K
• 年卷期：v.48,no.6(March 18, 2014)
• ISSN：1520-5851

文摘

Populations of Noccaea caerulescens show tremendous differences in their capacity to hyperaccumulate and hypertolerate metals. To explore the differences that could contribute to these traits, we undertook SOLiD high-throughput sequencing of the root transcriptomes of three phenotypically well-characterized N. caerulescens accessions, i.e., Ganges, La Calamine, and Monte Prinzera. Genes with possible contribution to zinc, cadmium, and nickel hyperaccumulation and hypertolerance were predicted. The most significant differences between the accessions were related to metal ion (di-, trivalent inorganic cation) transmembrane transporter activity, iron and calcium ion binding, (inorganic) anion transmembrane transporter activity, and antioxidant activity. Analysis of correlation between the expression profile of each gene and the metal-related characteristics of the accessions disclosed both previously characterized (HMA4, HMA3) and new candidate genes (e.g., for nickel IRT1, ZIP10, and PDF2.3) as possible contributors to the hyperaccumulation/tolerance phenotype. A number of unknown Noccaea-specific transcripts also showed correlation with Zn²⁺, Cd²⁺, or Ni²⁺ hyperaccumulation/tolerance. This study shows that N. caerulescens populations have evolved great diversity in the expression of metal-related genes, facilitating adaptation to various metalliferous soils. The information will be helpful in the development of improved plants for metal phytoremediation.