Self-Aligned Supported Lipid Bilayers for Patterning the Cell−Substrate Interface

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• 作者：Keyue Shen ; Jones Tsai ; Peng Shi ; Lance C. Kam
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：September 23, 2009
• 年：2009
• 卷：131
• 期：37
• 页码：13204-13205
• 全文大小：155K
• 年卷期：v.131,no.37(September 23, 2009)
• ISSN：1520-5126

文摘

Supported lipid bilayers capture the fluidity and chemical properties of cellular membranes. In this report, we introduce a method for creating surfaces that contain multiple, aligned regions of supported membranes of different compositions at scales of micrometers and smaller. This method uses the design of a diffusional barrier to increase the resolution that can be achieved directly using traditional bilayer patterning techniques, such as laminar flow. We demonstrate the use of this platform for presenting ligands to the T Cell Receptor and LFA-1 that are tethered to separate, closely juxtaposed regions of bilayer, capturing an important aspect of the natural organization observed between T cells and Antigen Presenting Cells. Our results present a novel platform for the study of spatial separation of extracellular ligands and its impact on cell signals.