Glutathione Responsive Hyaluronic Acid Nanocapsules Obtained by Bioorthogonal Interfacial “Click” Reaction

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• 作者：Grit Baier ; Michael Fichter ; Andreas Kreyes ; Katja Klein ; Volker Mail?nder ; Stephan Gehring ; Katharina Landfester
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：January 11, 2016
• 年：2016
• 卷：17
• 期：1
• 页码：148-153
• 全文大小：376K
• ISSN：1526-4602

文摘

Azide-functionalized hyaluronic acid and disulfide dialkyne have been used for “click” reaction polymerization at the miniemulsion droplets interface leading to glutathione responsive nanocapsules (NCs). Inverse miniemulsion polymerization was chosen, due to its excellent performance properties, for example, tuning of size and size distribution, shell thickness/density, and high pay loading efficiency. The obtained size, size distribution, and encapsulation efficiency were checked via fluorescent spectroscopy, and the tripeptide glutathione was used to release an encapsulated fluorescent dye after cleavage of the nanocapsules shell. To show the glutathione-mediated intracellular cleavage of disulfide-containing NC shells, CellTracker was encapsulated into the nanocapsules. The cellular uptake in dendritic cells and the cleavage of the nanocapsules in the cells were studied using confocal laser scanning microscopy. Because of the mild reaction conditions used during the interfacial polymerization and the excellent cleavage properties, we believe that the synthesis of glutathione responsive hyaluronic acid NCs reported herein are of high interest for the encapsulation and release of sensitive compounds at high yields.