2D Difference Gel Electrophoresis of Prepubertal and Pubertal Rat Mammary Gland Proteomes

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• 作者：Helen Kim ; Mark B. Cope ; Richie Herring ; Gloria Robinson ; Landon Wilson ; Grier P. Page ; Stephen Barnes
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：November 7, 2008
• 年：2008
• 卷：7
• 期：11
• 页码：4638-4650
• 全文大小：302K
• 年卷期：v.7,no.11(November 7, 2008)
• ISSN：1535-3907

文摘

Rat mammary gland proteomes at day 21 (prepubertal) and day 50 (late puberty) were compared by 2D difference gel electrophoresis. Two-hundred fifty-one spots were significantly different (p < 0.05) in abundance. Peptide mass fingerprint analysis of a subset of these proteins identified two significantly over-represented classes including structural and blood proteins (increased), and metabolism-relevant proteins (reduced) in day 50 relative to day 21 glands. This is a first report of mammary gland proteome differences at these important breast cancer-relevant time-points.