Sesquiterpene, Alkene, and Alkane Hydrocarbons in Virgin Olive Oils of Different Varieties and Geographical Origins

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• 作者：Renzo Bortolomeazzi ; Pierpaolo Berno ; Lorena Pizzale ; and Lanfranco S. Conte
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2001
• 出版时间：July 2001
• 年：2001
• 卷：49
• 期：7
• 页码：3278 - 3283
• 全文大小：71K
• 年卷期：v.49,no.7(July 2001)
• ISSN：1520-5118

文摘

The hydrocarbon fraction of 30 virgin olive oils was analyzed, focusing in particular on thesesquiterpenes. The oil samples were of different geographical origins and obtained from differentolive varieties. The hydrocarbon fraction was isolated by silica gel column chromatography of theunsaponifiable fraction of the oils. The sesquiterpene hydrocarbons were then fractionated, on thebasis of their degree of unsaturation, by AgNO₃ TLC and silica gel AgNO₃ column chromatography.The composition of the sesquiterpenes was more complex than previously reported. Among the 31sesquiterpenes detected, 24 have been tentatively identified, by comparison of the linear retentionindices on two capillary columns of different polarities and mass spectra with those reported in theliterature. The total concentration of the sesquiterpenes in the oils analyzed ranged from about 2to 37 ppm. Among the sesquiterpenes the more abundant were s/gifchars/alpha.gif" BORDER=0>-farnesene, s/gifchars/alpha.gif" BORDER=0>-copaene, eremophyllene, and s/gifchars/alpha.gif" BORDER=0>-muurolene. The alkenes present in the hydrocarbon fraction were isolated by TLC AgNO₃and characterized by GC-MS of their dimethyl disulfide derivatives. The series of n-s/gifchars/Delta.gif" BORDER=0 >⁹-alkenes fromC₂₂ to C₂₇, 8-heptadecene, and 6,10-dimethyl-1-undecene were detected. Among the n-alkanes, thosewith an odd number of carbon atoms predominated in all of the analyzed oils, the most commonbeing C₂₃, C₂₅, C₂₇, and C₂₉. The concentration of the n-alkenes ranged from about 0.5 to 2 ppm,whereas for the n-alkanes the range was from 30 to 177 ppm.Keywords: Virgin olive oil; hydrocarbons; sesquiterpenes; alkanes; alkenes