Detection of Halofuginone Residues in Chicken Liver Tissue by HPLC and a Monoclonal-Based Immunoassay

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• 作者：Ross C. Beier ; Terry J. Dutko ; Sandra A. Buckley ; Mark T. Muldoon ; Carol K. Holtzapple ; and Larry H. Stanker
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1998
• 出版时间：March 1998
• 年：1998
• 卷：46
• 期：3
• 页码：1049 - 1054
• 全文大小：82K
• 年卷期：v.46,no.3(March 1998)
• ISSN：1520-5118

文摘

The quinazolinone halofuginone (Hal) is a feed additive used worldwideto prevent coccidiosis incommercial poultry production. The current regulatory method fordetermining the action level ofHal residues in poultry involves measuring parent Hal in liver tissueby HPLC. That procedure isnot amenable to high sample throughput due to a complex and tedioussample preparation scheme.A competitive enzyme-linked immunosorbent assay (cELISA) that canbe used as a screening toolfor determining Hal in chicken liver tissue is described. ThecELISA method was evaluated usingstandard curves made in both assay buffer and chicken liver extract.The results demonstratedthat standard curves made in assay buffer could be used for the cELISA.HPLC vs cELISA resultswere obtained during two studies; the first study used spiked chickenliver tissue, and the secondstudy used both spiked chicken liver tissue and incurred levels of Halin chicken liver tissue. Therewas good agreement in the results obtained by HPLC and cELISA.However, in most cases therecovery was higher using the cELISA method than with the HPLC method.In addition, the cELISAmethod does not require the use of organic solvents. These dataclearly demonstrate that the cELISAmethod could be used as a screening method for the analysis of Hal inchicken liver tissue.Keywords: Broilers; chicken liver tissue; coccidiostat; ELISA;halofuginone; HPLC; immunoassay