Thousand-Fold Volumetric Concentration of Live Cells with a Recirculating Acoustofluidic Device

详细信息    查看全文

• 作者：Ola Jakobsson ; Seung Soo Oh ; Maria Antfolk ; Michael Eisenstein ; Thomas Laurell ; H. Tom Soh
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：August 18, 2015
• 年：2015
• 卷：87
• 期：16
• 页码：8497-8502
• 全文大小：329K
• ISSN：1520-6882

文摘

The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 渭L min^鈥?) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability. Importantly, our device could be readily adopted to serve as a 鈥渟ample preparation鈥?module that can be integrated with other microfluidic devices to allow analysis of dilute cellular samples in large volumes.