Studies on the bioavailability of contaminants thataccumulate in sediments have been complicated by thechemical and structural variability of substrates and by thedifferent biological properties of test organisms that areused by regulators. The purpose of this work was to overcomesome of these difficulties by devising a test system thatused artificial particles with known chemical surfaces. Thesewere coated with 2,4-dichlorophenol or pentachlorophenoland fed to oligochete worms (Lumbriculus variegatus)and midge larvae (Chironimus riparius). The adsorptioncoefficient (Kd) of the particle surface was compared withthe concentration of contaminant accumulated by thetest organisms. There were major differences in bioaccumulation between the two species used despite identicalparticles and pollutants. This clearly reflects differences inthe uptake and detoxification pathways between species.The particle surface and its interaction with the chlorophenols was a major factor in the accumulation of thecontaminants in an organism. The techniques thatare described provide a way of standardizing resultsbetween different natural sediments and different testorganisms and provide some insights into the processesinvolved in bioaccumulation from particle surfaces.