Copper Complex-Assisted DNA Hybridization
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- 作者：Gapian Bianké ; Valé ; rie Chaurin ; Maxim Egorov ; Jacques Lebreton ; Edwin C. Constable ; Catherine E. Housecroft ; Robert Hä ; ner
- 刊名：Bioconjugate Chemistry
- 出版年：2006
- 出版时间：November 2006
- 年：2006
- 卷：17
- 期：6
- 页码：1441 - 1446
- 全文大小：281K
- 年卷期：v.17,no.6(November 2006)
- ISSN：1520-4812
文摘
2,2'-Bipyridine (bpy) or 1,10-phenanthroline (phen) metal-binding domains were covalently attached tooligonucleotides, and the influence of metal ions on the hybridization of the conjugates was investigated. Metal-binding domains were attached to oligonucleotides at 3'- and 5'-terminal positions, thus placing them in juxtaposedpositions after hybridization to a common target strand. While the ligands alone had a positive effect (increasedTm) on hybrid stability, the duplex was further stabilized by the addition of copper(I) and/or copper(II) throughthe formation of a metal complex in which the two short sequences are linked through {Cu(bpy)2}, {Cu(phen)2},or {Cu(bpy)(phen)} domains. The increase in Tm, due to formation of the {Cu(bpy)2}, {Cu(phen)2}, or {Cu(bpy)(phen)} motifs is reversed upon addition of EDTA, consistent with the stripping of copper from the ligands.The effect of metal complex formation on the duplex strength was shown to be highest if the two metal-coordinatingligand strands are placed as close to each other as possible.