Stamp Wound Assay for Studying Coupled Cell Migration and Cell Debris Clearance

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• 作者：Jiyeon Lee ; Yu-Lin Wang ; Fan Ren ; Tanmay P. Lele
• 刊名：Langmuir
• 出版年：2010
• 出版时间：November 16, 2010
• 年：2010
• 卷：26
• 期：22
• 页码：16672-16676
• 全文大小：1065K
• 年卷期：v.26,no.22(November 16, 2010)
• ISSN：1520-5827

文摘

A new method for studying wound healing under realistic conditions in vitro was developed. The method involves creating defined patterns of damaged cell debris with poly(dimethyl)siloxane (PDMS) stamping. This novel assay permitted the quantification of wound healing rates in the presence of cell debris. Experimental results with this assay suggest that cell migration in the presence of cell debris is a two step process requiring (1) non-muscle myosin II-dependent cell clearance followed by (2) cell migration into newly cleared wound areas. The novel stamp wound assay allows the study of coupled cell migration and debris clearance and is a more realistic wound healing assay in vitro.