Independent versus Cooperative Binding in Polyethylenimine鈥揇NA and Poly(l-lysine)鈥揇NA Polyplexes

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• 作者：Tiia-Maaria Ketola ; Martina Hanzl铆kov谩 ; Linda Lepp盲nen ; Manuela Ravi帽a ; Corey J. Bishop ; Jordan J. Green ; Arto Urtti ; Helge Lemmetyinen ; Marjo Yliperttula ; Elina Vuorimaa-Laukkanen
• 刊名：Journal of Physical Chemistry B
• 出版年：2013
• 出版时间：September 12, 2013
• 年：2013
• 卷：117
• 期：36
• 页码：10405-10413
• 全文大小：392K
• 年卷期：v.117,no.36(September 12, 2013)
• ISSN：1520-5207

文摘

The mechanism of polyethylenimine鈥揇NA and poly(l-lysine)鈥揇NA complex formation at pH 5.2 and 7.4 was studied by a time-resolved spectroscopic method. The formation of a polyplex core was observed to be complete at approximately N/P = 2, at which point nearly all DNA phosphate groups were bound by polymer amine groups. The data were analyzed further both by an independent binding model and by a cooperative model for multivalent ligand binding to multisubunit substrate. At pH 5.2, the polyplex formation was cooperative at all N/P ratios, whereas for pH 7.4 at N/P < 0.6 the polyplex formation followed independent binding changing to cooperative binding at higher N/Ps.