Isoelectric Point Determination of Live Polioviruses by Capillary Isoelectric Focusing with Whole Column Imaging Detection

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• 作者：Yvonne E. Thomassen ; Gerco van Eikenhorst ; Leo A. van der Pol ; Wilfried A. M. Bakker
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：June 18, 2013
• 年：2013
• 卷：85
• 期：12
• 页码：6089-6094
• 全文大小：268K
• 年卷期：v.85,no.12(June 18, 2013)
• ISSN：1520-6882

文摘

Using a capillary isoelectric focusing鈥搘hole column imaging detection (CIEF-WCID) method, the isoelectric points (pI) of complete intact polioviruses were determined. The polioviruses that were analyzed are the commonly used viruses for the production of inactivated polio vaccines (IPV)鈥擬ahoney (type 1), MEF (type 2), and Saukett (type 3)鈥攁s well as for attenuated oral polio vaccines (OPV) and Sabin types 1, 2, and 3. A method for analyzing biological hazardous components (biological safety level 2) was set up for the CIEF-WCID analyzer used. This method is based on closed circuits. The determined pI鈥檚 were 6.2 for Mahoney, 6.7 for MEF-1, and 5.8 for Saukett. The pI鈥檚 of Sabin types 1, 2, and 3 viruses were 7.4, 7.2, and 6.3, respectively. Resolution of the virus peaks was shown to be reproducible. Using this adjusted CIEF-WCID technique, the pI of biologically hazardous components like toxins or viruses can be determined, which is beneficial for the development of vaccine production methods among others.