Effects of the Antithyroid Agent Propylthiouracil in a Partial Life Cycle Assay with Zebrafish

详细信息    查看全文

• 作者：Leo T. M. van der Ven ; Evert-Jan van den Brandhof ; José ; H. Vos ; Deborah M. Power ; and Piet W. Wester
• 刊名：Environmental Science & Technology
• 出版年：2006
• 出版时间：January 1, 2006
• 年：2006
• 卷：40
• 期：1
• 页码：74 - 81
• 全文大小：839K
• 年卷期：v.40,no.1(January 1, 2006)
• ISSN：1520-5851

文摘

Some ubiquitous pollutants of the aquatic environment,such as PCBs or other polyhalogenated aromatichydrocarbons, may disrupt the thyroid hormone system. Ina partial life cycle assay with zebrafish (Danio rerio), westudied the effects of the reference compound propylthiouracil(PTU) on reproduction, growth and development, histopathology of some target tissues, and plasma thyroid hormonelevels. PTU induced a concentration-dependent increaseof egg production with a concomitant decrease of matureoocyte size but had no effect on fertilization rate orhatching. In F1, serious dysmorphogenesis was found in 4dph larvae at the highest PTU level tested (100 mg/L),and there was a dose-dependent decrease in body lengthand weight at 42 dph (significant at 100 mg/L PTU). Atthis time, there was also a decreased scale thickness,suggesting inhibited metamorphosis, detectable at 1 mg/LPTU and higher. PTU also induced activation of thethyroid follicles in a concentration-dependent way, injuveniles associated with hyperemia in the thyroid area,and depletion of liver glycogen. Effects in adults wereassociated with decreased circulating levels of the thyroidhormones T₃ and T₄. These observations indicate thatdisruption of the thyroid hormone system may affect thefitness of these aquatic organisms. The zebrafish model maycontribute to the identification of thyroid hormonedisrupting activity in water samples and also in theinterpretation of histological observations in free-rangingfish species.