The
conversion of protein
mono
mers into fibrils
can be deter
mined using the
centrifugal filtration
method. Theresults of this
method were used to
calibrate steady-shear birefringen
ce and Thioflavin T fluores
cen
ce
measure
ments.For both
measure
ments, a linear
correlation with the fibril
con
centration was extra
cted, resulting in two fast assaysto deter
mine the fibril
con
centration quantitatively. Fro
m birefringen
ce
measure
ments and the
conversion deter
minedusing the
centrifugal filtration
method, we were able to
cal
culate
more pre
cise values for the birefringen
ce per unitlength of the fibrils (
M) and the flexibility of the fibrils (
![](/i<font color=)
mages/gif
chars/beta2.gif" BORDER=0 ALIGN="
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