Bacillus thuringiensis cry3Ca1 Protein Is Toxic to the Colorado Potato Beetle, Leptinotarsa decemlineata (Say)
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- 作者：Yosr Z. Haffani ; Conrad Cloutier ; and Franç ; ois J. Belzile
- 刊名：Biotechnology Progress
- 出版年：2001
- 出版时间：April 2001
- 年：2001
- 卷：17
- 期：2
- 页码：211 - 216
- 全文大小：85K
- 年卷期：v.17,no.2(April 2001)
- ISSN：1520-6033
文摘
We expressed the wild-type cry3Aa3 and cry3Ca1 Bacillus thuringiensis genes, whichcode for insecticidal proteins, in an Escherichia coli expression system. Highly purifiedpreparations of the soluble 
-endotoxins were used to perform comparative bioassayswith third-instar larvae of the Colorado potato beetle (CPB). Acute mortality datashowed that Cry3Ca1 (LD50 = 320.1 ng) was 2-fold more toxic than Cry3Aa3 (LD50 =672.9 ng). We also compared the chronic effects of sublethal doses of these toxins bymeasuring the consumption of untreated foliage and monitoring survival and development for 6 days after intoxication. No significant additional mortality was recorded,but we found that surviving larvae fed Cry3Ca1 consumed foliage at a slower ratethan the larvae fed Cry3Aa3, suggesting more damage to their digestive epithelium.This study, the first assessment of the toxicity of cry3Ca1 in third-instar CPB, suggestscry3Ca1 will prove useful for the control of this important insect pest.
-endotoxins were used to perform comparative bioassayswith third-instar larvae of the Colorado potato beetle (CPB). Acute mortality datashowed that Cry3Ca1 (LD50 = 320.1 ng) was 2-fold more toxic than Cry3Aa3 (LD50 =672.9 ng). We also compared the chronic effects of sublethal doses of these toxins bymeasuring the consumption of untreated foliage and monitoring survival and development for 6 days after intoxication. No significant additional mortality was recorded,but we found that surviving larvae fed Cry3Ca1 consumed foliage at a slower ratethan the larvae fed Cry3Aa3, suggesting more damage to their digestive epithelium.This study, the first assessment of the toxicity of cry3Ca1 in third-instar CPB, suggestscry3Ca1 will prove useful for the control of this important insect pest.