Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging

详细信息    查看全文

• 作者：Ling Zhu ; Kun Wang ; Jian Cui ; Huan Liu ; Xiangli Bu ; Huailei Ma ; Weizhi Wang ; He Gong ; Christopher Lausted ; Leroy Hood ; Guang Yang ; Zhiyuan Hu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：September 2, 2014
• 年：2014
• 卷：86
• 期：17
• 页码：8857-8864
• 全文大小：403K
• ISSN：1520-6882

文摘

Exosomes are endosome-derived membrane vesicles carrying proteins and nucleic acids that are involved in cellular functions such as intercellular communication, protein and RNA secretion, and antigen presentation. Therefore, exosomes serve as potential biomarkers for many diseases including cancer. Because exosomes are difficult to enrich or purify from biofluids, quantification of exosomes is tedious and inaccurate. Here, we present a real-time, label-free, and quantitative method to detect and characterize tumor-derived exosomes without enrichment or purification. Utilizing surface plasmon resonance imaging (SPRi) in combination with antibody microarrays specific to the extracellular domains of exosome membrane proteins, exosomes in tumor cell culture medium can be quantitatively detected. We found a positive correlation between the metastatic potential of tumor cell lines and exosome secretion. This method provides an easy, efficient, and novel way to detect exosome secretion and thus an avenue toward the diagnosis and prognosis prediction of cancer.