Thermodynamic Evidence for Ca2+-Mediated Self-Aggregation of Lewis X Gold Glyconanoparticles. A Model for Cell Adhesion via Carbohydrate-Carbohydrate Interaction

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• 作者：Jesú ; s M. de la Fuente ; Peter Eaton ; Africa G. Barrientos ; Margarita Mené ; ndez ; and Soledad Penadé ; s
• 刊名：Journal of the American Chemical Society
• 出版年：2005
• 出版时间：May 4, 2005
• 年：2005
• 卷：127
• 期：17
• 页码：6192 - 6197
• 全文大小：343K
• 年卷期：v.127,no.17(May 4, 2005)
• ISSN：1520-5126

文摘

Thermodynamic evidence for the selective Ca²⁺-mediated self-aggregation via carbohydrate-carbohydrate interactions of gold glyconanoparticles functionalized with the disaccharides lactose (lacto-Au) and maltose (malto-Au), or the biologically relevant trisaccharide Lewis X (Le^X-Au), was obtained byisothermal titration calorimetry. The aggregation process was also directly visualized by atomic forcemicroscopy. It was shown in the case of the trisaccharide Lewis X that the Ca²⁺-mediated aggregation isa slow process that takes place with a decrease in enthalpy of 160 ± 30 kcal mol^-1, while the heat evolvedin the case of lactose and maltose glyconanoparticles was very low and thermal equilibrium was quicklyachieved. Measurements in the presence of Mg²⁺ and Na⁺ cations confirm the selectivity for Ca²⁺ of Le^X-Au glyconanoparticles. The relevance of this result to cell-cell adhesion process mediated by carbohydrate-carbohydrate interactions is discussed.